A Novel Bacteriocin Against Shigella flexneri From Lactiplantibacillus plantarum Isolated From Tilapia Intestine: Purification, Antibacterial Properties and Antibiofilm Activity

A Novel Bacteriocin Against Shigella flexneri From Lactiplantibacillus plantarum Isolated From Tilapia Intestine: Purification, Antibacterial Properties and Antibiofilm Activity

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Few bacteriocins with antibacterial activity against Shigella flexneri have been reported.Here, a novel bacteriocin (LFX01) produced by Lactiplantibacillus plantarum strain LF-8 from the caruso rhodiola intestine of tilapia was purified and extensively characterized.LFX01 possesses a molecular weight of 1049.

56 Da and an amino acid sequence of I-T-G-G-P-A-V-V-H-Q-A.LFX01 significantly inhibited S.flexneri strain 14 (S.

flexneri_14) growth.Moreover, it exhibited excellent stability under heat and acid-base stress, and presented sensitivity to a variety of proteases, such as proteinase K, pepsin, and trypsin.The minimum inhibitory concentration (MIC) of LFX01 against S.

flexneri_14 was 12.65 μg/mL, which was smaller than that of most of the previously found bacteriocins.Furthermore, LFX01 significantly inhibited (p < 0.

05) S.flexneri_14 cells and decreased their remtavares.com cell viability.In addition, LFX01 could significantly (p < 0.

05) inhibit biofilm formation of S.flexneri_14.Scanning electron microscopy analysis presented that the cell membrane permeability of S.

flexneri_14 was demolished by LFX01, leading to cytoplasmic contents leakage and cell rupture death.In summary, a novel bacteriocin of lactic acid bacteria (LAB) was found, which could effectively control S.flexneri in both planktonic and biofilm states.